Fig. 3. Co-localization of NDRG2 with specific cell marker in immunofluorescence staining. Exemplary immunofluorescence staining of the following marker to define specific cell types: GFAP as marker for astrocytes (A), ChAT as marker for motor neurons (B) and CD68 as marker for microglial cells (C). A clear co-localization is visible for ChAT and NDRG2, whereas the other marker show nearly no co-localization. White areas in the merge channel represent a co-localization of both markers used. Nuclei were stained with DAPI (blue). Pictures were taken using a confocal laser scanning microscope (LSM 800, Zeiss, Germany with a 20x and a 40x objective (PlanApo 20x/0.8 DICII and PlanApo 40x/1.4 Oil DICII, Nikon instruments ). White arrows indicate co-localized signals.